In this article:

A column method,  which is applicable to coarse cellular isolates of the plant cell wall type, is described.

Coarse particles are held in the void spaces of a column made from 100 µ spherical glass beads. In this state the embedded material is subjected to a variety of defined solutions that are pumped at constant rate through the column.

The properties of an insoluble Β-fructofuranosidase from grape berries were evaluated in this manner as a demonstration of the scope of this method. Some comparisons with the normal test tube technique were made.

The possibility of complete automation and the potential of the method are briefly discussed.

A Column Method for Enzymic Characterization of Coarse Cellular Fractions: Application to Insoluble β-Fructofuranosidase from Grape

1965, Archives of Biochemistry and Biophysics, vol 113, issue 2, month 2, pages 451 – 456

csiro 060

Please note accessing PDF may be slow, thank you.