In this article:
Both soluble invertase and insoluble invertase were obtained from sultana grape berries homogenized under a variety of conditions.
Treatment of the insoluble fraction with berate buffe or non-ionic detergents solubilized most of the invertase.
Homogenates of grape berries prepared in the presence of Carbowax 4000, non-ionic detergents or protein (bovine serum albumin) contained up to 100 per cent of the total invertase in the soluble form.
It is suggested that insoluble invertase from grapes is an artifact of extraction caused by the formation of a tannin protein complex and/or a protein-tannin cellwall complex. Invertase in grapes is probably located in the cytoplasm or vacuoles of cells.
In contrast to grapes, insoluble invertase from aged aseptic carrot disks or from corn coleoptiles could not be solubilized.
Insoluble Invertase from Grapes: An Artifact of Extraction
1969, Phytochemistry, vol 8, pages 337 – 344
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