In this article:

Protoplasts isolated from callus cultures of the pericarp of Vitis vinifera cv.

Sultana regenerated cell walls within 3 days, when cultured in semi-solid media adjusted to 0.4 – 0.5 osmolal. Up to 12% of the cells divided during the next 7 days. A lower proportion passed through two division cycles.

Culture of Protoplasts from Grapevine Pericarp Callus

1974, Australian Journal of Plant Physiology, vol 1, pages 371-376

csiro 336

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