In this article:
The responses of grapevine varieties and hybrids, with a range of field disease resistance. to infection by downymildew were studied in vitro.
Dual cultures of host and pathogen were established for several grapevine varieties by inoculating sterile rooted shoot cultures with a contaminant free fungal spore isolate.
It was demonstrated that resistance to downy mildew was expressed in vitro, and a scale of infection, based on foliar symptom development 10 days after inoculation, was described to rank the varieties.
The order of ranking of the varieties correlated with field and glasshouse assessments.
This report demonstrates that the dual culture technique can be used as a reproducible screening procedure, independent of season, to select for host resistance to an obligate parasite.
Development of Methods for Screening Grapevines for Resistance to Infection by Downy Mildew .I . Dual Culture In Vitro
1986, American Journal of Enology and Viticulture, vol 37, issue 1, pages 61-66
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